Polynucleotide phosphorylase in ribosomes from Escherichia coli
نویسندگان
چکیده
منابع مشابه
Purification and properties of polynucleotide phosphorylase from Escherichia coli.
A method for the purification of polynucleotide phosphorylase from Escherichia coli has been developed. The purified enzyme has a specific activity 700-fold higher than the crude extract. When enzyme fractions obtained at different stages of the purification were assayed by phosphorolysis of polyadenylic acid (poly A) or 32P-orthophosphate exchange with the S’diphosphates of adenosine, uridine,...
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The Escherichia coli polynucleotide phosphorylase (PNPase; encoded by pnp), a phosphorolytic exoribonuclease, posttranscriptionally regulates its own expression at the level of mRNA stability and translation. Its primary transcript is very efficiently processed by RNase III, an endonuclease that makes a staggered double-strand cleavage about in the middle of a long stem-loop in the 5'-untransla...
متن کاملRegulation of Escherichia coli polynucleotide phosphorylase by ATP.
Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, processively catalyzes the phosphorolysis of RNA, releasing nucleotide diphosphates, and the reverse polymerization reaction. In Escherichia coli, both reactions are implicated in RNA decay, as addition of either poly(A) or heteropolymeric tails targets RNA to degradation. PNPase may also be associ...
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Polynucleotide phosphorylase (PNPase), an enzyme conserved in Bacteria and eukaryotic organelles, processively catalyzes the phosphorolysis of RNA releasing nucleotide diphosphates and the reverse polymerization reaction. In E. coli both reactions are implicated in RNA decay, as addition of either poly(A) or heteropolymeric tails targets RNA to degradation. PNPase may also be associated with th...
متن کاملIsolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
Three strains of Escherichia coli with altered polynucleotide phosphorylase, Q7, Q13, and Q27, were isolated by screening clones from heavily mutagenized cultures for low levels of the enzyme. The three mutations were found to cotransduce with argG and asp, and the pnp locus which they define was mapped with respect to these loci. An explanation for the nonreciprocal cotransduction frequencies ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1961
ISSN: 0006-2936
DOI: 10.1042/bj0810319